Sotol (Dasylirion cedrosanum Trel.) is a perennial species with numerous grayish-to-green leaves that grow symmetrically from the base of the stem outward. Its inflorescences can measure up to 3 m in height and contain membranous bracts that enclose seeds. It is a species that has been scarcely studied at the cytogenetic level, with only one report available in the literature. In Mexico, it has economic importance because it is used to prepare the alcoholic beverage sotol. In the present work, the chromosomes of Dasylirion cedrosanum Trel. were obtained and analyzed using different cytogenetic banding techniques and morphometric analysis to construct the rst karyotype for this species. Chromosomes were obtained by germinating plant seeds collected in the locality of Las Adjuntas, Santiago, Nuevo León, Mexico. Treatment with colchicine as an antimitotic was performed, followed by enzymatic treatment with …