This study investigated the impact of high-intensity exercise on DNA damage. A former high-performance athlete was compared with individuals who performed moderate exercise, evaluating genetic damage using the comet single-cell gel electrophoresis assay in blood samples. The results indicated greater DNA damage in the former athlete, suggesting a possible relationship between exercise intensity and genetic damage. However, due to the limited sample size, larger studies are required to confirm these findings and determine whether factors such as type of sport or other variables could influence the results.