Introduction Professional quality of life (QoPL) is the balance of demand and employment/personal resources. It is important to relate QoPL to the pathological state of professional burnout, a product of the mismatch between demands of the position and personal capabilities. The questionnaires on QoPL and burnout, Spanish version self-administered format have not been validated. Objective To validate the psychometric properties of the scale, and to perform a factor analysis and construct validity of the Mexican Spanish version of the Professional Quality of Life questionnaires CVP-35 and the Maslach Inventory MBI-HSS using Internet surveys. Method Observational, prospective, cross-sectional, multicentre, and analytical design. Participants : Medical residents on a national medical specialty or subspecialty course, 2015-2016 at ISSSTE. Those with illness and psychiatric treatment were excluded. All subjects were informed of the study and gave their consent to participate. They received via e-mail with completion and shipping instructions for June-July 2015. Results Of the 360 responses received from 19 locations nationwide, 17 were excluded due to responding more than once (n = 338). Validation CVP-35: 35 5-point Likert scale items. Reliability: α = .93. Principal component analysis: KMO = .9. Bartlett sphericity test P < .001. Factors: 8 with eigenvalues > 1 explained 62.5% of total variance (job demands, management support, intrinsic motivation, support equipment, institutional feedback, perceived quality of life, physical and personal demands, financial support). Validation MBI-HSS: 22 Likert scale items 7 points, α = .885, KMO = .886. Bartlett sphericity test P < .001. Factors: 3 (emotional exhaustion, personal accomplishment, depersonalisation) with eigenvalues > 1 accounted for 51.17% of total variance. Pearson correlation coefficient was calculated. Significant > .3. Correlations emotional exhaustion of MBI-HSS domains correlated positively with CVP-35 job demands r = .399 (P < .0001) and poorer quality of life perceived r = .409 (P < .0001). Personal accomplishment MBI-HSS correlated with intrinsic motivation of the CVP-35 r = .379 (P < .0001). Conclusions Both instruments (CVP-35 and MBI-HSS) demonstrated reliability and validity in their online self-administered format in medical residents.