nfectious diseases remain a public health problem that is exacerbated by the presence of resistant organisms leading todevelopment of novel drugs. Study of pharmacological proteins from natural sources recently emerged. In this researchextraction of proteins from gel of Aloe vera and antimicrobial evaluation were done. Protein extracts from Aloe vera wereobtained by two methods: extract 1(EP1) by precipitation with (NH 4)2SO4 and (EP2) by phenol extraction. The yield formethod 2 (0.33 % recovery). Analysis by mass spectrometry of EP1 identied with a high degree of condence, peptide 5and 31-kDa protein, whose identity has been associated with the glycoproteins of Arabidopsis thaliana. EP2 exhibitedinhibitory activity against Staphylococcus aureus and Candida albicans. A. vera extract comprises a promising source ofprotein with anti-S. aureus and anti-C. albicans activity