Ovarian failure and postmenopausic usually coincide with aging in women. At this time isn’t known if the hormonal changes observed in several endocrine systems such as adrenal and somatotropic axis are conditioned by estrogens deficiency or are independents. Hormone replacement therapy (HRT) can reverse biochemical and organic alterations of menopause, nevertheless the repercussion on other endocrine systems are still in controversy. To demonstrate that hypoestrogenism in menopause is in part responsible for the aging of the endocrine systems and to analyse the relationship between estradiol (E2) levels with changes in the hormonal concentration of adrenal (ACTH, cortisol and DHEA-S) and somatotropic (GH, IGF-1) axis, as well with prolactin and insulin. In addition we studied the effect of HRT on all these hormones. Sixty women with spontaneous menopause aged 45-60 years were included in the study. Women showed elevated baseline serum levels of FSH and LH (> 50 mUI/mL) coinciding with hypoestrogenism. (E2 < 30 pg/mL). Participants were divided in two groups in order to receive a daily dose of 0.312 and 0.625 mg of conjugated equine estrogens (CEE) respectively. Hormone levels of FSH, LH, ACTH, GH, Prolactin DHEA-S, Cortisol, insulin-like growth factor (IGF-1) and insulin were analysed before and after three cycles of CEE for 21 days fallowed each by chormandinone acetate. Before treatment levels of ACTH, cortisol, DHEA-S, GH, IGF-1 and prolactin were found diminished and increased significantly after three months of HRT. In contrast elevated baseline levels of both gonadotropins (FSH, LH) and insulin decreased when women received estrogens. In addition a highly significant correlation was found between estradiol and all hormonal levels and the end of HRT. Treatment with EEC increased ACTH, GH, PRL, DHEA-S, cortisol and IGF-1 concentrations to values which may suggest that this therapy may exert a simultaneous and stimulatory effect in the pituitary gland, adrenal cortex and somatotropic axis, when baseline hipoestrogenism is present.