Hexanic and methanolic extracts from aerial parts of T. vulgaris inhibited the growth of M. tuberculosis H37Rv. Bioassay-guided from the most active extract to led obtained secondary fraction with antimicobacterial activity. From the secondary fractions active compounds were obtained and were identified by spectrometric and spectroscopic data. The mixture of (E and Z)-phytol and ursolic and oleanolic acid results active against M. tuberculosis H37Rv (MIC=25 µg/mL) and moderately active against MDR M. tuberculosis clinical isolates with MIC values from 6.25 to 12.5 µg/mL. Main compounds from T. vulgaris, thymol, carvacrol and mixture of them were inactive against M. tuberculosis H37Rv. Both extracts showing LD50 >5g/kg and IC50 = 646 ppm. Mixture of thymol/carvacrol and ursolic/oleanolic acid exhibited the IC50 = 250 and >1000 ppm, respectively. Key words: Thymus vulgaris, antimycobacterial compounds, MDR M. tuberculosis